Rabbit Transforming Growth factor beta1, TGF-beta1 ELISA Kit | MyBioSource MBS165545 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit Transforming Growth factor beta1, TGF-beta1 ELISA Kit

catalog :

MBS165545

quantity :

48-Strip-Wells

price :

280 USD

product information

catalog number :

MBS165545

products type :

ELISA Kit

products full name :

Rabbit Transforming Growth factor beta1, TGF-beta1 ELISA Kit

products short name :

[Transforming Growth factor beta1, TGF-beta1]

other names :

[transforming growth factor beta-1 preproprotein; Transforming growth factor beta-1; transforming growth factor beta-1; transforming growth factor, beta 1]

products gene name :

[TGF-beta1]

other gene names :

[Tgfb1; Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1; TGF-beta-1; LAP]

reactivity :

Rabbit

sequence length :

390

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5pg/ml - 2000pg/ml. Sensitivity: 2.56pg/ml

other info2 :

Intra-Assay: CV<8% . Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rabbit Transforming Growth Factor Beta 1 (also known as TGF-B1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit TGF-B1 antibody. TGF-B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit TGF-B1 Antibody is added and binds to TGF-B1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TGF-B1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit TGF-B1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

6755775

ncbi acc num :

NP_035707.1

ncbi gb acc num :

NM_011577.2

uniprot acc num :

P04202

ncbi mol weight :

44,310 Da

ncbi pathways :

AGE-RAGE Signaling Pathway In Diabetic Complications (1320037); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Adipogenesis Pathway (198299); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Cell Cycle Pathway (83251); Cell Cycle Pathway (198407); Cell Cycle Pathway (463); Cell Cycle Signaling Pathway (522974); Chagas Disease (American Trypanosomiasis) Pathway (147810)

ncbi summary :

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016]

uniprot summary :

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (PubMed:22781750). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types ().

size1 :

48-Strip-Wells

price1 :

280 USD

size2 :

96-Strip-Wells

price2 :

445

size3 :

5x96-Strip-Wells

price3 :

1830

size4 :

10x96-Strip-Wells

price4 :

3580