Human Large Multifunctional Peptidase 7 (LMP7) ELISA Kit | MyBioSource MBS165247 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Large Multifunctional Peptidase 7 (LMP7) ELISA Kit

catalog :

MBS165247

quantity :

48-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS165247

products type :

ELISA Kit

products full name :

Human Large Multifunctional Peptidase 7 (LMP7) ELISA Kit

products short name :

[Large Multifunctional Peptidase 7 (LMP7)]

other names :

[LMP7; Proteasome subunit beta type-8; proteasome subunit beta type-8; proteasome subunit beta 8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting new gene 10 protein]

products gene name :

[LMP7]

other gene names :

[PSMB8; PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E; LMP7; PSMB5i; RING10; Y2]

reactivity :

Human

sequence length :

272

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.1ng/ml - 40ng/ml. Sensitivity: 0.04ng/ml

other info2 :

Intra-Assay: CV<8% . Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Large Multifunctional Peptidase 7 (also known as LMP7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human LMP7 antibody. LMP7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human LMP7 Antibody is added and binds to LMP7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LMP7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human LMP7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

34637

ncbi acc num :

CAA47026.1

uniprot acc num :

P28062

ncbi mol weight :

29,770 Da

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1016198); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1016193); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1016194); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); AUF1 (hnRNP D0) Destabilizes MRNA Pathway (187212); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1016197); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1016195)

ncbi summary :

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]

uniprot summary :

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

395

size3 :

5x96-Strip-Wells

price3 :

1610

size4 :

10x96-Strip-Wells

price4 :

3135

more info or order :

MyBioSource product webpage