ELISA/assay

Human adrenomedullin, ADM ELISA Kit

MBS164826

48-Strip-Wells

260 USD

ELISA Kit

Human adrenomedullin, ADM ELISA Kit

[adrenomedullin, ADM]

[adrenomedullin; ADM; ADM; adrenomedullin; Adrenomedullin; AM]

[ADM]

[ADM; ADM; AM; PAMP; AM; AM; PAMP; ProAM-N20]

Human

185

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ng/L - 1500ng/L. Sensitivity: 2.52ng/L

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Adrenomedullin (also known as ADM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ADM antibody. ADM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ADM Antibody is added and binds to ADM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ADM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

765330

AAC60642.1

P35318

20,420 Da

Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); HIF-1-alpha Transcription Factor Network Pathway (138045); Myometrial Relaxation And Contraction Pathways (198759); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135