ELISA/assay

Human Semaphorin 3C (SEMA3C) ELISA Kit

MBS164777

48-Strip-Wells

260 USD

ELISA Kit

Human Semaphorin 3C (SEMA3C) ELISA Kit

[Semaphorin 3C (SEMA3C)]

[semaphorin-3C isoform 2; Semaphorin-3C; semaphorin-3C; semaphorin 3C; Semaphorin-E; Sema E]

[SEMA3C]

[SEMA3C; SEMA3C; SemE; SEMAE; SEMAE; Sema E]

Human

751

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml-200ng/ml. Sensitivity: 0.25ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Selenoprotein-P (also known as SE-P) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SE-P antibody. SE-P present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SE-P Antibody is added and binds to SE-P in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SE-Pantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SE-P. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

5454048

NP_006370.1

NM_006379.4

Q99985

26,255 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476)

This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017]

Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance ().

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135