ELISA/assay

Mouse C-telopeptide of type I collagen, CTX-I ELISA Kit

MBS164705

48-Strip-Wells

280 USD

ELISA Kit

Mouse C-telopeptide of type I collagen, CTX-I ELISA Kit

[C-telopeptide of type I collagen, CTX-I]

[Cytotoxin 1; Cytotoxin 1; Cardiotoxin 1; CTX-1; CTX1; Cardiotoxin A1]

[CTX-I]

[CTX A12 PublicationsManual assertion based on opinion iniRef.7; CTX I]

Mouse

81

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.02ng/ml - 1ng/ml. Sensitivity: 0.01ng/ml

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse C-telopeptide of Type II Collagen (also known as CTX-I) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CTX-I antibody. CTX-I present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CTX-I Antibody is added and binds to CTX-I in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CTX-I antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CTX-I. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

41688809

P60304.1

P60304

8,992 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities on many other cells, including red blood cells. Interaction with sulfatides in the cell membrane induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation (). It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity and inhibits protein kinases C (PubMed:8448165). It also binds with high affinity to heparin (PubMed:17685633). It also causes skeletal muscle necrosis after intramuscular injection into mice (PubMed:8342169).

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580