ELISA/assay

Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit

MBS164640

48-Strip-Wells

280 USD

ELISA Kit

Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit

[neuronal nitric oxide synthase, NOS, nNOS]

[neuronal nitric oxide synthase; Nitric oxide synthase, brain; nitric oxide synthase, brain; nitric oxide synthase 1; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS]

[nNOS]

[NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS]

Rat

1554

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.3ng/ml-90ng/ml. Sensitivity: 0.14ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Neuromedin U (also known as NMU) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat NMU antibody. NMU present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NMU Antibody is added and binds to NMU in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NMU antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NMU. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

642518

AAB60654.1

P29475

148,919 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Circadian Entrainment Pathway (698773)

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580