ELISA/assay

Human Interleukin 2, IL-2 ELISA Kit

MBS164623

48-Strip-Wells

260 USD

ELISA Kit

Human Interleukin 2, IL-2 ELISA Kit

[Interleukin 2, IL-2]

[interleukin-2; Interleukin-2; interleukin-2; interleukin 2; T-cell growth factor; TCGF; INN: Aldesleukin]

[IL-2]

[IL2; IL2; IL-2; TCGF; lymphokine; IL-2; TCGF]

Human

153

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/L-100ng/L. Sensitivity: 0.02ng/L

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Interleukin 18 (also known as IL-18) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human IL-18 antibody. IL-18 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human IL-18 Antibody is added and binds to IL-18 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-18 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IL-18. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

512412

CAA01199.1

P60568

17,628 Da

AhR Pathway (755436); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171)

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135