ELISA/assay

Human bone Alkaline Phosphatase, BALP ELISA Kit

MBS163950

48-Strip-Wells

260 USD

ELISA Kit

Human bone Alkaline Phosphatase, BALP ELISA Kit

[bone Alkaline Phosphatase, BALP]

[BALP]

Human

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml-200ng/ml. Sensitivity: 0.23ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Bisphenol A (also known as BPA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human BPA antibody. BPA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human BPA Antibody is added and binds to BPA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BPAantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human BPA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135