catalog number :
MBS163936
products type :
ELISA Kit
products full name :
Human Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit
products short name :
[Secreted Frizzled Related Protein 4 (SFRP4)]
other names :
[secreted frizzled-related protein 4; Secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled related protein 4; Frizzled protein, human endometrium; FrpHE]
products gene name :
[SFRP4]
other gene names :
[SFRP4; SFRP4; PYL; FRP-4; FRPHE; FRZB-2; sFRP-4; FRPHE; sFRP-4; FrpHE]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.05ng/ml - 15ng/ml. Sensitivity: 0.024ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled Related Protein 4 (also known as SFRP4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SFRP4 antibody. SFRP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SFRP4 Antibody is added and binds to SFRP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SFRP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
NP_003005.2
ncbi gb acc num :
NM_003014.3
ncbi mol weight :
39,827 Da
ncbi pathways :
Adipogenesis Pathway (198832); Wnt Signaling Pathway (198789); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)
ncbi summary :
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]
uniprot summary :
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed:12952927).
size4 :
10x96-Strip-Wells