ELISA/assay

Human glyceraldehyde-3-phosphate dehydrogenase, GAPDH ELISA Kit

MBS163595

48-Strip-Wells

260 USD

ELISA Kit

Human glyceraldehyde-3-phosphate dehydrogenase, GAPDH ELISA Kit

[glyceraldehyde-3-phosphate dehydrogenase, GAPDH]

[Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)]

[GAPDH]

[LOC108351137; Gapdh; Gapd; GAPDH]

Human

333

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml - 70ng/ml. Sensitivity: 0.23ng/ml

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Glyceraldehyde-3-phosphate Dehydrogenase (also known as GAPDH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GAPDH antibody. GAPDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GAPDH Antibody is added and binds to GAPDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAPDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GAPDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

122065190

P04797.3

P04797

35,828 Da

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Androgen Receptor Signaling Pathway (198529); Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (434661); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (434659)

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation ().

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135