ELISA/assay

Human High Mobility Group Protein B1, HMGB-1 ELISA Kit

MBS162952

48-Strip-Wells

260 USD

ELISA Kit

Human High Mobility Group Protein B1, HMGB-1 ELISA Kit

[High Mobility Group Protein B1, HMGB-1]

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; High mobility group protein 1; HMG-1]

[HMGB-1]

[HMGB1; HMGB1; HMG-1; HMG1; HMG-1]

Human

215

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 20ng/L-4500ng/L. Sensitivity: 13.17ng/L

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human High Mobility Group Protein 17 (also known as HMG-17) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HMG-17 antibody. HMG-17 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HMG-17 Antibody is added and binds to HMG-17 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HMG-17 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HMG-17. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

126352708

NP_001075304.1

NM_001081835.2

Q08IE6

24,894 Da

Base Excision Repair Pathway (89996); Base Excision Repair Pathway (451)

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135