ELISA/assay

Porcine Cathepsin B, cath-B ELISA Kit

MBS162934

48-Strip-Wells

335 USD

ELISA Kit

Porcine Cathepsin B, cath-B ELISA Kit

[Cathepsin B, cath-B]

[cathepsin B isoform 1 preproprotein; Cathepsin B; cathepsin B; cathepsin B; APP secretase; APPS]

[cath-B]

[CTSB; CTSB; APPS; CPSB; RECEUP; CPSB; APPS]

Porcine

339

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.3ng/ml - 90ng/ml. Sensitivity: 0.147ng/ml

Intra-Assay: CV<8%. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Cathepsin B (also known as Cath-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine Cath-B antibody. Cath-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine Cath-B Antibody is added and binds to Cath-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Cath-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine Cath-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

22538437

NP_680093.1

NM_147783.3

P07858

37,822 Da

Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Apoptosis Pathway (83060); Apoptosis Pathway (470); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1270247); Collagen Degradation Pathway (1270259); Collagen Formation Pathway (1270245); Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244)

This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Nov 2015]

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

48-Strip-Wells

335 USD

96-Strip-Wells

470

5x96-Strip-Wells

1945

10x96-Strip-Wells

3800