Mouse Laminin, LN ELISA Kit | MyBioSource MBS162395 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Laminin, LN ELISA Kit

catalog :

MBS162395

quantity :

48-Strip-Wells

price :

280 USD

product information

catalog number :

MBS162395

products type :

ELISA Kit

products full name :

Mouse Laminin, LN ELISA Kit

products short name :

[Laminin, LN]

other names :

[Laminin; Laminin subunit gamma-2; laminin subunit gamma-2; laminin subunit gamma 2; Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; Laminin B2t chain; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma]

products gene name :

[LN]

other gene names :

[LAMC2; LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2; LAMB2T; LAMNB2; CSF 140 kDa subunit]

reactivity :

Mouse

sequence length :

1111

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml - 200ng/ml. Sensitivity: 0.25ng/ml

other info2 :

Intra-Assay: CV<8%. Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Laminin (also known as LN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse LN antibody. LN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LN Antibody is added and binds to LN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse LN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

34232

ncbi acc num :

CAA78729.1

uniprot acc num :

Q13753

ncbi mol weight :

121,604 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478)

ncbi summary :

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]

uniprot summary :

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

size1 :

48-Strip-Wells

price1 :

280 USD

size2 :

96-Strip-Wells

price2 :

445

size3 :

5x96-Strip-Wells

price3 :

1830

size4 :

10x96-Strip-Wells

price4 :

3580