ELISA/assay

Human tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit

MBS162352

48-Strip-Wells

260 USD

ELISA Kit

Human tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit

[tartrate-resistant acid phosphatase 5b, TRACP-5b]

[TRACP-5b]

Human

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 20ng/L-6000ng/L. Sensitivity: 10.49ng/L

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human TAR DNA Binding Protein 43 (also known as TARDBP/TDP-43) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human TARDBP/TDP-43 antibody. TARDBP/TDP-43 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human TARDBP/TDP-43 Antibody is added and binds to TARDBP/TDP-43 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TARDBP/TDP-43 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human TARDBP/TDP-43. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135