ELISA/assay

Human Endostatin, ES ELISA Kit

MBS161858

48-Strip-Wells

260 USD

ELISA Kit

Human Endostatin, ES ELISA Kit

[Endostatin, ES]

[endostatin variant, partial; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen type XVIII alpha 1 chain]

[ES]

[COL18A1; COL18A1; KS; KNO; KNO1; NC1]

Human

102

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.3ng/ml - 90ng/ml. Sensitivity: 0.15ng/ml

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Endostatin (also known as ES) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ES antibody. ES present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ES Antibody is added and binds to ES in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ES antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ES. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

13957730

AAK50626.1

P39060

154,018 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Degradation Of Collagen Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (576262); FOXA1 Transcription Factor Network Pathway (137979); Protein Digestion And Absorption Pathway (172847)

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135