catalog number :
MBS161801
products type :
ELISA Kit
products full name :
Rat Neuron-specific enolase, NSE ELISA Kit
products short name :
[Neuron-specific enolase, NSE]
other names :
[neuron-specific enolase; Neuron-specific enolase; gamma-enolase; Neuron-specific enolase]
products gene name :
[NSE]
other gene names :
[ENO2; NSE; NSE]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.2ng/ml - 60ng/ml. Sensitivity: 0.09ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Neuron-specific enolase (also known as NSE) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat NSE antibody. NSE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NSE Antibody is added and binds to NSE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NSE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NSE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
AEV53911.1
ncbi mol weight :
47,266 Da
ncbi pathways :
Biosynthesis Of Amino Acids Pathway (916003); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (916000); Carbon Metabolism Pathway (817567); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (918797); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (918795); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (468194); Glycolysis / Gluconeogenesis Pathway (915918); Glycolysis / Gluconeogenesis Pathway (287)
size4 :
10x96-Strip-Wells