ELISA/assay

Mouse growth differentiation factor 15, GDF15 ELISA Kit

MBS161755

48-Strip-Wells

280 USD

ELISA Kit

Mouse growth differentiation factor 15, GDF15 ELISA Kit

[growth differentiation factor 15, GDF15]

[growth/differentiation factor 15 preproprotein; Growth/differentiation factor 15; growth/differentiation factor 15; growth differentiation factor 15; Macrophage inhibitory cytokine 1]

[GDF15]

[GDF15; GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; NAG-1; NRG-1]

Mouse

308

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 10ng/L - 1000ng/L. Sensitivity: 6.12ng/L

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Growth Differentiation Factor 15 (also known as GDF15) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse GDF15 antibody. GDF15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GDF15 Antibody is added and binds to GDF15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GDF15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GDF15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

153792495

NP_004855.2

NM_004864.3

Q99988

34,140 Da

Direct P53 Effectors Pathway (137939)

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:23468844, PubMed:29046435). Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed:28953886). On hepatocytes, inhibits growth hormone signaling ().

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580