Human Mucin-1, MUC1 ELISA Kit | MyBioSource MBS161679 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Mucin-1, MUC1 ELISA Kit

catalog :

MBS161679

quantity :

48-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS161679

products type :

ELISA Kit

products full name :

Human Mucin-1, MUC1 ELISA Kit

products short name :

[Mucin-1, MUC1]

other names :

[mucin-1 isoform 10; Mucin-1; mucin-1; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3]

products gene name :

[MUC1]

other gene names :

[MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta]

reactivity :

Human

sequence length :

484

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.05ng/ml - 15ng/ml. Sensitivity: 0.024ng/ml

other info2 :

Intra-Assay: CV<8% . Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Mucin-1 (also known as MUC1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MUC1 antibody. MUC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MUC1 Antibody is added and binds to MUC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MUC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MUC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

324120958

ncbi acc num :

NP_001191215.1

ncbi gb acc num :

NM_001204286.1

uniprot acc num :

P15941

ncbi mol weight :

122,941 Da

ncbi pathways :

IL-7 Signaling Pathway (198857); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

ncbi summary :

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

uniprot summary :

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.MiscellaneousThe name KL-6 was originally that of a murine monoclonal antibody reacting with pulmonary adenocarcinoma cell lines and pulmonary epithelial cells. This antibody recognizes a sialylated carbohydrate chain on MUC1.CautionO-glycosylation sites are annotated in first sequence repeat only. Residues at similar position are probably glycosylated in all repeats. Experimental sites were determined in a synthetic peptide glycosylated in vitro (PubMed:7744025, PubMed:9597769).

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

395

size3 :

5x96-Strip-Wells

price3 :

1610

size4 :

10x96-Strip-Wells

price4 :

3135

more info or order :

MyBioSource product webpage