Porcine tumor necrosis factor alpha, TNF-alpha ELISA Kit | MyBioSource MBS161499 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine tumor necrosis factor alpha, TNF-alpha ELISA Kit

catalog :

MBS161499

quantity :

48-Strip-Wells

price :

335 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS161499

products type :

ELISA Kit

products full name :

Porcine tumor necrosis factor alpha, TNF-alpha ELISA Kit

products short name :

[tumor necrosis factor alpha, TNF-alpha]

other names :

[TNF-alpha; Tumor necrosis factor; tumor necrosis factor; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a]

products gene name :

[TNF-alpha]

other gene names :

[TNF; TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2]

reactivity :

Porcine

sequence length :

233

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 20ng/L-7000ng/L. Sensitivity: 10.25ng/L

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine transforming growth factor beta (also known as TGF-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine TGF-B antibody. TGF-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine TGF-B Antibody is added and binds to TGF-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TGF-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine TGF-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

37210

ncbi acc num :

CAA26669.1

uniprot acc num :

P01375

ncbi mol weight :

25,644 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); AhR Pathway (755436); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509)

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

uniprot summary :

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).

size1 :

48-Strip-Wells

price1 :

335 USD

size2 :

96-Strip-Wells

price2 :

470

size3 :

5x96-Strip-Wells

price3 :

1945

size4 :

10x96-Strip-Wells

price4 :

3800

more info or order :

MyBioSource product webpage