catalog number :
MBS161476
products type :
ELISA Kit
products full name :
Human Vasoactive Intestinal Peptide, VIP ELISA Kit
products short name :
[Vasoactive Intestinal Peptide, VIP]
other names :
[vasoactive intestinal peptide, partial; VIP peptides; VIP peptides; vasoactive intestinal peptide; Intestinal peptide PHV-42Alternative name(s):Peptide histidine valine 42]
products gene name :
[VIP]
other gene names :
[VIP; VIP; PHM27; VIP]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 20ng/L-3800ng/L. Sensitivity: 9.81ng/L
other info2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Ubiquitin Specific Peptidase 18 (also known as USP18) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human USP18 antibody. USP18 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human USP18 Antibody is added and binds to USP18 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated USP18 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human USP18. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
AAA63268.1
ncbi mol weight :
19,082 Da
ncbi pathways :
G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)
ncbi summary :
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]
uniprot summary :
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
size4 :
10x96-Strip-Wells