catalog number :
MBS161471
products type :
ELISA Kit
products full name :
Rat macrophage inflammatory protein 1beta, MIP-1beta ELISA Kit
products short name :
[macrophage inflammatory protein 1beta, MIP-1beta]
other names :
[MIP-1beta; C-C motif chemokine 4 homolog; C-C motif chemokine 4 homolog; Macrophage inflammatory protein 1-beta homolog; MIP-1-beta; Small-inducible cytokine A4 homolog]
products gene name :
[MIP-1beta]
other gene names :
[CCL4; CCL4; SCYA4; MIP-1beta; SCYA4; MIP-1-beta]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 2ng/L - 600ng/L. Sensitivity: 0.08ng/L
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Macrophage Inflammatory Protein 1 Beta (also known as MIP-1B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MIP-1B antibody. MIP-1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MIP-1B Antibody is added and binds to MIP-1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MIP-1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MIP-1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
ABO77445.1
ncbi mol weight :
9,969 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125152); Cytosolic DNA-sensing Pathway (124833); Salmonella Infection Pathway (375175); Salmonella Infection Pathway (375149); Toll-like Receptor Signaling Pathway (117308); Toll-like Receptor Signaling Pathway (487)
uniprot summary :
Monokine with inflammatory and chemokinetic properties.
size4 :
10x96-Strip-Wells