ELISA/assay

Human Vitamin D-binding protein, DBP ELISA Kit

MBS161429

48-Strip-Wells

260 USD

ELISA Kit

Human Vitamin D-binding protein, DBP ELISA Kit

[Vitamin D-binding protein, DBP]

[vitamin D-binding protein isoform 1; Vitamin D-binding protein; vitamin D-binding protein; GC, vitamin D binding protein; Gc protein-derived macrophage activating factor]

[DBP]

[GC; GC; DBP; VDB; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; DBP-maf; HEL-S-51; Gc-MAF; GcMAF1 PublicationManual assertion based on opinion iniRef.15; Gc1 PublicationManual assertion based on opinion iniRef.15; DBP-maf]

Human

474

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 1ng/ml-300ng/ml. Sensitivity: 0.52ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Vitamin C (also known as VC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human VC antibody. VC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human VC Antibody is added and binds to VC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human VC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

324021743

NP_001191235.1

NM_001204306.1

P02774

39,496 Da

Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Vitamin D (calciferol) Metabolism Pathway (160956); Vitamin D Synthesis Pathway (198764)

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

48-Strip-Wells

260 USD

96-Strip-Wells

395

5x96-Strip-Wells

1610

10x96-Strip-Wells

3135