catalog number :
MBS161232
products type :
ELISA Kit
products full name :
Human heme oxygenase 1, HO-1 ELISA Kit
products short name :
[heme oxygenase 1, HO-1]
other names :
[heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heme oxygenase 1]
products gene name :
[HO-1]
other gene names :
[HMOX1; HMOX1; HO-1; HSP32; HMOX1D; bK286B10; HO; HO1; HO-1]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.1ng/ml - 9ng/ml. Sensitivity: 0.05ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Heme Oxygenase 1 (also known as HO-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HO-1 antibody. HO-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HO-1 Antibody is added and binds to HO-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HO-1antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HO-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
NP_002124.1
ncbi gb acc num :
NM_002133.2
ncbi mol weight :
32,819 Da
ncbi pathways :
HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Heme Degradation Pathway (160971); Iron Uptake And Transport Pathway (187191); Keap1-Nrf2 Pathway (198777); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Oxidative Stress Pathway (198916)
ncbi summary :
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
uniprot summary :
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
size4 :
10x96-Strip-Wells