catalog number :
MBS161118
products type :
ELISA Kit
products full name :
Porcine Interleukin 1beta, IL-1beta ELISA Kit
products short name :
[Interleukin 1beta, IL-1beta]
other names :
[interleukin 1, beta]
products gene name :
[IL-1beta]
other gene names :
[IL1B2; IL1B; IL-1beta]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ug/ml-1000ug/ml. Sensitivity: 2.41ug/ml
other info2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Immunoglobulin E (also known as IgE) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IgE antibody. IgE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IgE Antibody is added and binds to IgE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IgE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IgE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
NP_001005149.1
ncbi gb acc num :
NM_001005149.1
ncbi pathways :
African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Apoptosis Pathway (84472); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795)
size4 :
10x96-Strip-Wells