ELISA/assay

Rat inducible nitric oxide synthase, iNOS/NOS2 ELISA Kit

MBS161069

48-Strip-Wells

280 USD

ELISA Kit

Rat inducible nitric oxide synthase, iNOS/NOS2 ELISA Kit

[inducible nitric oxide synthase, iNOS/NOS2]

[inducible nitric oxide synthase; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2]

[iNOS/NOS2]

[Nos2; Inducible NOS; iNOS]

Rat

1147

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.2ng/ml-70ng/ml. Sensitivity: 0.081ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Hypoxanthine-guanine Phosphoribosyltransferase (also known as HPRT1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HPRT1 antibody. HPRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HPRT1 Antibody is added and binds to HPRT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HPRT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HPRT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

2935553

AAC83553.1

130,628 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580