catalog number :
MBS160948
products type :
ELISA Kit
products full name :
Rat brain natriuretic peptide, BNP ELISA Kit
products short name :
[brain natriuretic peptide, BNP]
other names :
[brain natriuretic peptide, partial; Natriuretic peptides B; natriuretic peptides B; natriuretic peptide B; Gamma-brain natriuretic peptide]
products gene name :
[BNP]
other gene names :
[NPPB; NPPB; BNP; BNP(1-32); BNP-32]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 1ng/L - 400ng/L. Sensitivity: 0.54ng/L
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Brain Natriuretic Peptide (also known as BNP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat BNP antibody. BNP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat BNP Antibody is added and binds to BNP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BNP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat BNP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
BAA04067.1
ncbi mol weight :
14,726 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
ncbi summary :
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
uniprot summary :
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
size4 :
10x96-Strip-Wells