catalog number :
MBS160864
products type :
ELISA Kit
products full name :
Rat Renin ELISA Kit
products short name :
[Renin]
other names :
[renin; Renin; Angiotensinogenase]
products gene name :
[Renin]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ng/L - 1000ng/L. Sensitivity: 2.49ng/L
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Renin (also known as renin) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat renin antibody. renin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat renin Antibody is added and binds to renin in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated renin antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat renin. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
AAA60363.1
ncbi mol weight :
44,726 Da
ncbi pathways :
ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)
uniprot summary :
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
size4 :
10x96-Strip-Wells