catalog number :
MBS160764
products type :
ELISA Kit
products full name :
Human Semaphorin 3A (SEMA3A) ELISA Kit
products short name :
[Semaphorin 3A (SEMA3A)]
other names :
[semaphorin-3A; Semaphorin-3A; semaphorin-3A; semaphorin 3A; Semaphorin III; Sema III]
products gene name :
[SEMA3A]
other gene names :
[SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.3ng/ml - 90ng/ml. Sensitivity: 0.14ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Semaphorin 3A (also known as Sema3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Sema3A antibody. Sema3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Sema3A Antibody is added and binds to Sema3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Sema3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Sema3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
NP_006071.1
ncbi gb acc num :
NM_006080.2
ncbi mol weight :
88,889 Da
ncbi pathways :
Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)
ncbi summary :
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]
uniprot summary :
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
size4 :
10x96-Strip-Wells