catalog number :
MBS160748
products type :
ELISA Kit
products full name :
Human Angiopoietin 2, ANG-2 ELISA Kit
products short name :
[Angiopoietin 2, ANG-2]
other names :
[angiopoietin-2 isoform c; Angiopoietin-2; angiopoietin-2; angiopoietin 2]
products gene name :
[ANG-2]
other gene names :
[ANGPT2; ANGPT2; ANG2; AGPT2; ANG-2]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ng/L - 1000ng/L. Sensitivity: 2.49ng/L
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Angiopoietin 2 (also known as ANG-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ANG-2 antibody. ANG-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ANG-2 Antibody is added and binds to ANG-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANG-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ANG-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
NP_001112360.1
ncbi gb acc num :
NM_001118888.1
ncbi mol weight :
50,958 Da
ncbi pathways :
Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Tie2 Signaling Pathway (106063)
ncbi summary :
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
size4 :
10x96-Strip-Wells