catalog number :
MBS160612
products type :
ELISA Kit
products full name :
Rat Hepcidin, Hepc ELISA Kit
products short name :
[Hepcidin, Hepc]
other names :
[hepcidin; Hepcidin; Hepcidin]
products gene name :
[Hepc]
other gene names :
[hepcidin]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 5ng/ml - 2000ng/ml. Sensitivity: 2.49ng/ml
other info2 :
Intra-Assay: CV<8% . Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Hepcidin (also known as HEPC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HEPC antibody. HEPC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HEPC Antibody is added and binds to HEPC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HEPC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HEPC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
CAZ68137.1
ncbi mol weight :
10,491 Da
size4 :
10x96-Strip-Wells