ELISA/assay

Rat Endothelial nitric oxide synthase, eNOS ELISA Kit

MBS160509

48-Strip-Wells

280 USD

ELISA Kit

Rat Endothelial nitric oxide synthase, eNOS ELISA Kit

[Endothelial nitric oxide synthase, eNOS]

[endothelial nitric oxide synthase; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; nitric oxide synthase 3; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII]

[eNOS]

[NOS3; NOS3; eNOS; ECNOS; cNOS; eNOS; NOSIII]

Rat

1203

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.6ng/ml-180ng/ml. Sensitivity: 0.35ng/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Elastin (also known as ELN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ELN antibody. ELN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ELN Antibody is added and binds to ELN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ELN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ELN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

825652

CAA53950.1

P29474

68,965 Da

ACE Inhibitor Pathway (198763); AGE/RAGE Pathway (698754); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Disease Pathway (530764); Endothelin Pathway (672453)

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580