catalog number :
MBS160436
products type :
ELISA Kit
products full name :
Rat adiponectin, ADP ELISA Kit
products short name :
[adiponectin, ADP]
other names :
[adiponectin; Adiponectin; Adiponectin]
products gene name :
[ADP]
other gene names :
[ADIPOQ]
storage stability :
Store at 2 to 8 degree C for 6 months.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.05ng/ml-10ng/ml. Sensitivity: 0.016ng/ml
other info2 :
Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
products description :
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Acetyl Coenzyme A (also known as Ac-COA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat Ac-COA antibody. Ac-COA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Ac-COA Antibody is added and binds to Ac-COA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Ac-COA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat Ac-COA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
ncbi acc num :
ADY38781.1
ncbi mol weight :
26,292 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells