Human Bruton tyrosine kinase, Btk ELISA Kit | MyBioSource MBS160192 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Bruton tyrosine kinase, Btk ELISA Kit

catalog :

MBS160192

quantity :

48-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS160192

products type :

ELISA Kit

products full name :

Human Bruton tyrosine kinase, Btk ELISA Kit

products short name :

[Bruton tyrosine kinase, Btk]

other names :

[Bruton's tyrosine kinase; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK; Bruton tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase]

products gene name :

[Btk]

other gene names :

[BTK; BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1; AGMX1; ATK; BPK; ATK; BPK]

reactivity :

Human

sequence length :

659

storage stability :

Store at 2 to 8 degree C for 6 months.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.05ng/ml - 35ng/ml. Sensitivity: 0.02ng/ml

other info2 :

Intra-Assay: CV<8% . Inter-Assay: CV<10%

products description :

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Bruton Tyrosine Kinase (also known as Btk) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Btk antibody. Btk present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Btk Antibody is added and binds to Btk in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Btk antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Btk. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

ncbi gi num :

1684918

ncbi acc num :

AAB64205.1

uniprot acc num :

Q06187

ncbi mol weight :

79,937 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway (576249); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Class I PI3K Signaling Events Pathway (138022); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

ncbi summary :

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

uniprot summary :

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

395

size3 :

5x96-Strip-Wells

price3 :

1610

size4 :

10x96-Strip-Wells

price4 :

3135

more info or order :

MyBioSource product webpage