ELISA/assay

Hamster lipoprotein lipase, LPL ELISA Kit

MBS1600791

48-Strip-Wells

385 USD

ELISA Kit

Hamster lipoprotein lipase, LPL ELISA Kit

[lipoprotein lipase, LPL]

[lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase]

[LPL]

[LPL; LPL; LIPD; HDLCQ11; LIPD; LPL]

Hamster

475

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 1ng/L-400ng/L. Sensitivity: 0.64ng/L

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Hamster Interleukin 8 (also known as IL-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Hamster IL-8 antibody. IL-8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Hamster IL-8 Antibody is added and binds to IL-8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Hamster IL-8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

4557727

NP_000228.1

NM_000237.2

P06858

53,162 Da

Adipogenesis Pathway (198832); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Chylomicron-mediated Lipid Transport Pathway (106157); Developmental Biology Pathway (477129); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986)

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL) (PubMed:27578112). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium ().

48-Strip-Wells

385 USD

96-Strip-Wells

505

5x96-Strip-Wells

2095

10x96-Strip-Wells

4095