ELISA/assay

Rat Autophagy related protein LC3 B (LC3B) ELISA Kit

MBS1600540

48-Strip-Wells

280 USD

ELISA Kit

Rat Autophagy related protein LC3 B (LC3B) ELISA Kit

[Autophagy related protein LC3 B (LC3B)]

[LC3B]

Rat

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 15ng/L-3000ng/L. Sensitivity: 7.69ng/L

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat ATP-binding Cassette Subfamily A Member 1 (also known as ABCA1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ABCA1 antibody. ABCA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ABCA1 Antibody is added and binds to ABCA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ABCA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

48-Strip-Wells

280 USD

96-Strip-Wells

445

5x96-Strip-Wells

1830

10x96-Strip-Wells

3580