ELISA/assay

Porcine cytosolic phospholipase A2, cPLA2 ELISA Kit

MBS160054

48-Strip-Wells

335 USD

ELISA Kit

Porcine cytosolic phospholipase A2, cPLA2 ELISA Kit

[cytosolic phospholipase A2, cPLA2]

[cytosolic phospholipase A2 isoform 1; Cytosolic phospholipase A2; cytosolic phospholipase A2; phospholipase A2 group IVA; Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase]

[cPLA2]

[PLA2G4A; PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha; CPLA2; PLA2G4; cPLA2]

Porcine

749

Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.3ng/ml - 90ng/ml. Sensitivity: 0.151ng/ml

Intra-Assay: CV<8% . Inter-Assay: CV<10%

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Cytosolic Phospholipase A2 (also known as CPLA2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates. Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine CPLA2 antibody. CPLA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CPLA2 Antibody is added and binds to CPLA2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CPLA2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine CPLA2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

23943920

NP_077734.1

NM_024420.2

P47712

85,239 Da

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); Acyl Chain Remodeling Of CL Pathway (645330); Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553)

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

48-Strip-Wells

335 USD

96-Strip-Wells

470

5x96-Strip-Wells

1945

10x96-Strip-Wells

3800