PDL1 Antibody [8E12] | MyBioSource MBS154571 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PDL1 Antibody [8E12]

catalog :

MBS154571

quantity :

0.1 mg

price :

440 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[8E12]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 g/ml

image 2 :

Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 g/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 g/mL.

image 3 :

Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 g/mL. Red: PDL1 Antibody [8E12]. Blue: DAPI staining

product information

catalog number :

MBS154571

products type :

Antibody

products full name :

PDL1 Antibody [8E12]

products short name :

[PDL1]

products name syn :

[Programmed cell death 1 ligand; Programmed cell death 1 ligand-1; programmed death ligand 1; PDL1; PDL-1; B7-H1]

other names :

[programmed cell death 1 ligand 1 isoform a; Programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; CD274 molecule; B7 homolog 1]

products gene name :

[PDL1]

products gene name syn :

[CD274]

other gene names :

[CD274; CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7-H1]

clonality :

Monoclonal

isotype :

IgG1

clone :

[8E12]

host :

Mouse

reactivity :

Human

sequence length :

290

purity :

Protein A purified

form :

Liquid. Supplied in PBS containing 0.02% sodium azide and 50% glycerol.

concentration :

1 mg/mL

storage stability :

Store at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

app notes :

WB at 0.25-0.5 ug/mL. IHC starting at 2-5 ug/mL. IF start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal diultions for each application to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunocytochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 g/mL. Red: PDL1 Antibody [8E12]. Blue: DAPI staining

image5 heading :

Immunofluorescence (IF)

image5 description :

Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 g/mL. Red: PDL1 Antibody [8E12]. Blue: DAPI staining

other info1 :

Immunogen: PD-L1 antibody was raised against the extracellular domain of human PD-L1. Conjugate: Unconjugated

other info2 :

Positive Control: Human Stomach Carcinoma Tissue Lysate. (Please inquire). Human Tonsil Tissue. (Please inquire)

products categories :

Immunology

products description :

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).

products references :

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78. Aydin AM, Woldu SL, Hutchinson RC, et al. Spotlight on atezolizumab and its potential in the treatment of advanced urothelial bladder cancer.Onco. Targets Ther. 2017;10:1487-502.

ncbi gi num :

7661534

ncbi acc num :

NP_054862

ncbi gb acc num :

NM_014143.3

uniprot acc num :

Q9NZQ7

ncbi mol weight :

Predicted: 32 kDa

ncbi pathways :

Adaptive Immune System Pathway (1269171); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (1269177); Immune System Pathway (1269170); PD-1 Signaling Pathway (1269182)

ncbi summary :

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

size1 :

0.1 mg

price1 :

440 USD

more info or order :

MyBioSource product webpage