P2RX7 Antibody | MyBioSource MBS153696 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

P2RX7 Antibody

catalog :

MBS153696

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 g/ml.

image 2 :

Immunocytochemistry of P2RX7 in 3T3 cells with P2RX7 antibody at 5 g/mL.

image 3 :

Immunofluorescence of P2RX7 in 3T3 cells with P2RX7 antibody at 20 g/mL.

product information

catalog number :

MBS153696

products type :

Antibody

products full name :

P2RX7 Antibody

products short name :

[P2RX7]

products name syn :

[P2X purinoceptor 7; P2X7; purinergic receptor P2X ligand-gated ion channel 7; purinergic receptor P2X, ligand-gated ion channel, 7]

other names :

[P2X purinoceptor 7; P2X purinoceptor 7; P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A; purinergic receptor P2X, ligand-gated ion channel, 7; purinergic receptor P2X, ligand gated ion channel, 7; ATP receptor; P2Z receptor; Purinergic receptor]

products gene name :

[P2RX7]

other gene names :

[P2RX7; P2RX7; P2X7; P2X7]

uniprot entry name :

P2RX7_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Reactivity: Human, Mouse. Predicted species reactivity based on immunogen sequence: Rat: (81%)

sequence length :

595

specificity :

P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.

purity :

P2RX7 antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

P2RX7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

tested application :

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 ug/ml. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Optimal dilutions for each application to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunocytochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Conjugate: Unconjugated. Immunogen: P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7. Buffer: P2RX7 antibody is supplied in PBS containing 0.02% sodium azide.

products description :

The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).

ncbi gi num :

300068987

ncbi acc num :

NP_002553

ncbi gb acc num :

NM_002562.5

uniprot acc num :

Q99572

ncbi mol weight :

Predicted: 34; 47; 65 kDa. Observed: 54 kDa

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Immune System Pathway (106386); Inflammasomes Pathway (366166); Innate Immune System Pathway (106387); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (366164); The NLRP3 Inflammasome Pathway (366167)

ncbi summary :

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

uniprot summary :

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation. Protein type: Channel, ligand-gated; Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: protein complex; membrane; cell soma; integral to plasma membrane; cytoplasm; plasma membrane; terminal button; integral to nuclear inner membrane; intercellular junction; neuromuscular junction; lipid raft; external side of plasma membrane. Molecular Function: protein binding; copper ion binding; protein homodimerization activity; zinc ion binding; lipopolysaccharide binding; magnesium ion binding; purinergic nucleotide receptor activity; ATP binding; receptor binding; ATP-gated cation channel activity. Biological Process: activation of MAPK activity; positive regulation of T cell mediated cytotoxicity; cytolysis; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; cell surface receptor linked signal transduction; multicellular organismal protein catabolic process; ceramide biosynthetic process; positive regulation of cytolysis; T cell homeostasis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; positive regulation of cytoskeleton organization and biogenesis; regulation of sodium ion transport; protein oligomerization; phagolysosome formation; phospholipid translocation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to mechanical stimulus; response to zinc ion; cell volume homeostasis; response to calcium ion; phospholipid transfer to membrane; generation of action potential; cell morphogenesis; regulation of killing of cells of another organism; T cell proliferation; plasma membrane organization and biogenesis; positive regulation of mitochondrial depolarization; pore complex biogenesis; inflammatory response; negative regulation of bone resorption; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; synaptic vesicle exocytosis; homeostasis of number of cells within a tissue; skeletal morphogenesis; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; innate immune response; gene expression; protein processing; membrane budding. Disease: Leukemia, Chronic Lymphocytic

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage