BHLHA15 Antibody | MyBioSource MBS153611 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BHLHA15 Antibody

catalog :

MBS153611

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 g/ml in (A) the absence and (B) the presence of blocking peptide

image 2 :

Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 g/mL.

image 3 :

Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 g/mL.

product information

catalog number :

MBS153611

products type :

Antibody

products full name :

BHLHA15 Antibody

products short name :

[BHLHA15]

products name syn :

[BHLHA15; MIST1; BHLHB8; MIST1; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; bHLHa15; basic helix-loop-helix family, member a15]

other names :

[class A basic helix-loop-helix protein 15; Class A basic helix-loop-helix protein 15; class A basic helix-loop-helix protein 15; MIST-1; class II bHLH protein MIST1; class B basic helix-loop-helix protein 8; muscle, intestine and stomach expression 1; basic helix-loop-helix domain containing, class B, 8; basic helix-loop-helix family, member a15; Class B basic helix-loop-helix protein 8; bHLHb8; Muscle, intestine and stomach expression 1; MIST-1]

products gene name :

[BHLHA15]

other gene names :

[BHLHA15; BHLHA15; MIST1; BHLHB8; BHLHB8; MIST1; bHLHa15; bHLHb8; MIST-1]

uniprot entry name :

BHA15_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence positions :

[60-110]

specificity :

BHLHA15 antibody is human, mouse and rat reactive.

purity :

BHLHA15 antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

BHLHA15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Conjugate: Unconjugated. Immunogen: BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. Buffer: BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide. Positive Control: Rat Small Intestine Tissue Lysate: MBS151680. Human small Intestine Tissue Slide: MBS154284. Homology: Predicted species reactivity based on immunogen sequence: Bovine: (71%)

products description :

BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).

ncbi gi num :

29126247

ncbi acc num :

NP_803238

ncbi gb acc num :

NM_177455.3

uniprot acc num :

Q7RTS1

ncbi mol weight :

Predicted: 21 kDa. Observed: 24 kDa

ncbi pathways :

Maturity Onset Diabetes Of The Young Pathway (83096); Maturity Onset Diabetes Of The Young Pathway (508)

uniprot summary :

MIST1: Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5 -CANNTG-3 . Chromosomal Location of Human Ortholog: 7q21.3. Cellular Component: nucleus. Molecular Function: protein homodimerization activity. Biological Process: G-protein coupled receptor protein signaling pathway; cellular response to glucose starvation; transcription, DNA-dependent; cell-cell signaling; unfolded protein response; cell maturation; intracellular distribution of mitochondria; calcium-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; Golgi organization and biogenesis; mitochondrial calcium ion transport

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage