ERAP1 Antibody | MyBioSource MBS153542 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ERAP1 Antibody

catalog :

MBS153542

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 g/ml.

image 2 :

Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 g/mL.

image 3 :

Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 g/mL.

product information

catalog number :

MBS153542

products type :

Antibody

products full name :

ERAP1 Antibody

products short name :

[ERAP1]

products name syn :

[ERAP1 Antibody : ALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP; KIAA0525; UNQ584/PRO1154; Endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase 1]

other names :

[endoplasmic reticulum aminopeptidase 1 isoform a; Endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase 1; aminopeptidase PILS; adipocyte-derived leucine aminopeptidase; aminopeptidase regulator of TNFR1 shedding; puromycin-insensitive leucyl-specific aminopeptidase; endoplasmic reticulum aminopeptidase 1 delta-Exon-11 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-13 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-14 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-15 isoform; endoplasmic reticulum aminopeptidase associated with antigen processing; type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator]

products gene name :

[ERAP1]

other gene names :

[ERAP1; ERAP1; ALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP; APPILS; ARTS1; KIAA0525; A-LAP; PILS-AP]

uniprot entry name :

ERAP1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

948

specificity :

ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.

purity :

ERAP1 Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

ERAP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)

app notes :

ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 mug/ml. Optimal dilutions for each application to be determined by the researcher.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Conjugate: Unconjugated. Immunogen: ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP. Buffer: ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide. Homology: Predicted species reactivity based on immunogen sequence: Rat: (78%). Positive Control: 1)MBS151737 SK-N-SH Cell lysate.

products description :

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).

ncbi gi num :

94818901

ncbi acc num :

NP_057526

ncbi gb acc num :

NM_016442.3

uniprot acc num :

Q9NZ08

ncbi mol weight :

Predicted: 104 kDa. Observed: 105kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386)

ncbi summary :

The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

uniprot summary :

ARTS1: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. Belongs to the peptidase M1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 3.4.11.-; Protease. Chromosomal Location of Human Ortholog: 5q15. Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; endoplasmic reticulum lumen; endoplasmic reticulum; integral to membrane; extracellular region; cytosol. Molecular Function: protein binding; interleukin-6 receptor binding; metalloexopeptidase activity; zinc ion binding; interleukin-1, Type II receptor binding; aminopeptidase activity. Biological Process: fat cell differentiation; positive regulation of angiogenesis; antigen processing and presentation of peptide antigen via MHC class I; response to bacterium; regulation of blood pressure; membrane protein ectodomain proteolysis; angiogenesis; antigen processing and presentation of endogenous peptide antigen via MHC class I; regulation of innate immune response

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage