PTRF Antibody | MyBioSource MBS153486 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PTRF Antibody

catalog :

MBS153486

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PTRF in human spleen tissue lysate with PTRF antibody at (A) 0.5 and (B) 1 g/mL

image 2 :

Immunohistochemistry of PTRF in human spleen tissue with PTRF antibody at 5 g/ml.

image 3 :

Immunofluorescence of PTRF in human spleen tissue with PTRF antibody at 20 g/ml.

product information

catalog number :

MBS153486

products type :

Antibody

products full name :

PTRF Antibody

products short name :

[PTRF]

products name syn :

[PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1; Polymerase I and transcript release factor; Cavin-1; polymerase I and transcript release factor]

other names :

[polymerase I and transcript release factor; Polymerase I and transcript release factor; polymerase I and transcript release factor; PTRF; TTF-I interacting peptide 12; RNA polymerase I and transcript release factor; polymerase I and transcript release factor; Cavin-1]

products gene name :

[PTRF]

other gene names :

[PTRF; PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1]

uniprot entry name :

PTRF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

390

specificity :

At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family.

purity :

PTRF Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

PTRF antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

app notes :

PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 mug/mL.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

other info1 :

Conjugate: Unconjugated. Immunogen: Rabbit polyclonal PTRF antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PTRF. Positive Control: Cat. No. MBS151674- Human Spleen Tissue Lysate

other info2 :

Buffer: PTRF Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

PTRF Antibody: The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

ncbi gi num :

42734430

ncbi acc num :

NP_036364

ncbi gb acc num :

NM_012232.5

uniprot acc num :

Q6NZI2

ncbi mol weight :

43 kDa

ncbi pathways :

Gene Expression Pathway (105937); RNA Polymerase I Transcription Pathway (106550); RNA Polymerase I Transcription Termination Pathway (106556); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948)

ncbi summary :

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

uniprot summary :

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription initiation complex. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: nucleoplasm; protein complex; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; cytosol; nucleus. Molecular Function: protein binding; rRNA primary transcript binding. Biological Process: regulation of transcription, DNA-dependent; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter. Disease: Lipodystrophy, Congenital Generalized, Type 4

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage