MyD88 Fragment Recombinant Protein | MyBioSource MBS153301 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MyD88 Fragment Recombinant Protein

catalog :

MBS153301

quantity :

0.05 mg

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

SDS-PAGE analysis of recombinant MyD88 fragment on Coomassie Blue-stained 4-20% gradient gel.

product information

catalog number :

MBS153301

products type :

Recombinant Protein

products full name :

MyD88 Fragment Recombinant Protein

products short name :

[MyD88]

products name syn :

[MyD88 Antibody : MYD88D; myeloid differentiation primary response gene (88)]

other names :

[MyD88; Myeloid differentiation primary response protein MyD88; myeloid differentiation primary response protein MyD88; myeloid differentiation primary response gene (88); myeloid differentiation primary response 88]

products gene name :

[MYD88]

other gene names :

[MYD88; MYD88; MYD88D]

uniprot entry name :

MYD88_HUMAN

host :

E. Coli

reactivity :

Human

sequence length :

296

sequence :

aa 176 - 280

purity :

~95%

form :

Liquid; 1X PBS containing 0.1% SDS and 0.02% NaN 3

storage stability :

Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. Shipping: Dry Ice.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

This recombinant protein can be used for WB and ELISA. For research use only.

image1 heading :

SDS-PAGE

other info1 :

Fusion Tag: Fusion Partner: His-tag and strepII-tag at N-terminus

products description :

The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-?B activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

ncbi gi num :

1814020

ncbi acc num :

AAC50954

uniprot acc num :

Q99836

ncbi mol weight :

15 kDa (Calculated)

ncbi pathways :

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (106400); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Modulation And Signaling Pathway (198822); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171)

ncbi summary :

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

uniprot summary :

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 3p22. Cellular Component: plasma membrane; endosome membrane; cytosol. Molecular Function: identical protein binding; protein binding; death receptor binding. Biological Process: positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; regulation of inflammatory response; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis. Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency

size1 :

0.05 mg

price1 :

270 USD

more info or order :

MyBioSource product webpage