SnoN Antibody | MyBioSource MBS151484 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

SnoN Antibody

catalog :

MBS151484

quantity :

0.1 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS151484

products type :

Antibody

products full name :

SnoN Antibody

products short name :

SnoN

products name syn :

SnoN; SNO; SnoA; SnoI; SnoN; SNO; Ski-like protein; Ski-related oncogene; SKI-like oncogene

other names :

ski-like protein isoform 1; Ski-like protein; ski-like protein; SKI-like oncogene; ski-related oncogene snoN; SKI-like proto-oncogene; Ski-related oncogene; Ski-related protein

products gene name :

SKIL

other gene names :

SKIL; SKIL; SNO; SnoA; SnoI; SnoN; SNO

uniprot entry name :

SKIL_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

684

purity :

SnoN Antibody is affinity chromatography purified via peptide column.

form :

Liquid

storage stability :

SnoN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

app notes :

SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

other info1 :

Conjugate: Unconjugated. Immunogen: SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN. Buffer: SnoN Antibody is supplied in PBS containing 0.02% sodium azide.

products description :

SnoN Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).

ncbi gi num :

223029418

ncbi acc num :

NP_005405

ncbi gb acc num :

NM_005414.4

uniprot acc num :

P12757

ncbi mol weight :

74,784 Da

ncbi pathways :

Disease Pathway (530764); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (645266); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Loss Of Function Of SMAD2/3 In Cancer Pathway (906019); Loss Of Function Of SMAD4 In Cancer Pathway (906017); Loss Of Function Of TGFBR1 In Cancer Pathway (906025); Loss Of Function Of TGFBR2 In Cancer Pathway (906022); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); SMAD2/3 MH2 Domain Mutants In Cancer Pathway (906021)

ncbi summary :

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 3q26. Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus. Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity. Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; response to antibiotic; regulation of apoptosis; negative regulation of cell differentiation; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; positive regulation of axonogenesis; gene expression; spermatogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; lymphocyte homeostasis

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage