PION Antibody | MyBioSource MBS151476 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PION Antibody

catalog :

MBS151476

quantity :

0.1 mg

price :

375 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of PION in EL4 cell lysate with PION antibody at 0.25 g/mL in (A) the absence and (B) the presence of blocking peptide.

image 2 :

Immunohistochemistry of PION in human brain tissue with PION antibody at 5 g/mL.

image 3 :

Immunofluorescence of PION in Human Brain cells with PION antibody at 20 g/mL.

product information

catalog number :

MBS151476

products type :

Antibody

products full name :

PION Antibody

products short name :

[PION]

products name syn :

[PION; PION; PION; Gamma-secretase-activating protein; Protein pigeon homolog; GSAP; pigeon homolog (Drosophila)]

other names :

[gamma-secretase-activating protein; Gamma-secretase-activating protein; gamma-secretase-activating protein; pigeon homolog; protein pigeon homolog; gamma-secretase activating protein; Protein pigeon homologGamma-secretase-activating protein 16 kDa C-terminal form; GSAP-16K]

products gene name :

[PION]

other gene names :

[GSAP; GSAP; PION; PION; GSAP; GSAP-16K]

uniprot entry name :

GSAP_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence positions :

[770-820]

sequence length :

820

specificity :

Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members.

purity :

PION Antibody is affinity chromatography purified via peptide column.

form :

Liquid

concentration :

1 mg/mL

storage stability :

PION antibody can be stored at 4°C for three months and -20° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

tested application :

ELISA, IF, IHC-P, WB

app notes :

PION antibody can be used for detection of PION by Western blot at 0.25 mg/mL. Antibody can also be used for immunohistochemistry starting at 5 mg/mL. For immunofluorescence start at 20 ug/mL.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of PION in mouse brain tissue with PION antibody at 5 g/mL.

image5 heading :

Immunofluorescence (IF)

image5 description :

Immunofluorescence of PION in mouse brain tissue with PION antibody at 20 g/mL. Red: PION Antibody (6161). Blue: DAPI staining

other info1 :

Conjugate: Unconjugated. Immunogen: PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.

other info2 :

Buffer: PION Antibody is supplied in PBS containing 0.02% sodium azide. Positive control: MBS151675- EL4 Cell Lysate. MBS154323- Human Brain Tissue Slide.

products description :

PION Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer's disease patient's brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease.

ncbi gi num :

148612805

ncbi acc num :

NP_059135

ncbi gb acc num :

NM_017439.3

uniprot acc num :

A4D1B5

ncbi summary :

Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]

uniprot summary :

GSAP: Regulator of gamma-secretase activity, which specifically activates the production of beta-amyloid protein (beta-amyloid protein 40 and beta-amyloid protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate beta- amyloid, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. Belongs to the GSAP family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Activator. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: trans-Golgi network. Molecular Function: beta-amyloid binding. Biological Process: regulation of proteolysis

size1 :

0.1 mg

price1 :

375 USD

more info or order :

MyBioSource product webpage