antibody

XBP-1 Antibody

MBS151369

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

XBP-1 Antibody

XBP-1

XBP-1; XBP2; TREB5; XBP-1; XBP2; X-box-binding protein 1; Tax-responsive element-binding protein 5; X-box binding protein 1

X-box-binding protein 1; X-box-binding protein 1; X-box-binding protein 1; tax-responsive element-binding protein 5; X-box binding protein 1; Tax-responsive element-binding protein 5

XBP1

XBP1; XBP1; XBP2; TREB5; XBP-1; TREB5; XBP2; XBP-1

XBP1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

261

XBP-1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

XBP-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)

XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.

Conjugate: Unconjugated. Immunogen: XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1. Buffer: XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

60416406

P17861.2

P17861

40,148 Da

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); FOXA1 Transcription Factor Network Pathway (137979); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); IRE1alpha Activates Chaperones Pathway (105906); Insulin Signaling Pathway (198845); Metabolism Of Proteins Pathway (106230); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314)

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5 -GATGACGTG[TG]N(3)[AT]T-3 , and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 22q12.1 22q12. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; nucleus; cytosol. Molecular Function: protein binding; protein homodimerization activity; protease binding; DNA binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; estrogen receptor binding; transcription factor activity; protein kinase binding. Biological Process: ubiquitin-dependent protein catabolic process; transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; muscle development; apoptosis; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; response to electrical stimulus; cell growth; positive regulation of autophagy; fatty acid biosynthetic process; positive regulation of TOR signaling pathway; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; positive regulation of immunoglobulin production; cholesterol homeostasis; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; positive regulation of T cell differentiation; fatty acid homeostasis; endothelial cell proliferation; positive regulation of B cell differentiation; positive regulation of fat cell differentiation; neuron development; autophagy; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; sterol homeostasis; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis. Disease: Major Affective Disorder 7

0.1 mg

345 USD