antibody

Spred1 Antibody

MBS151347

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Spred1 Antibody

Spred1

Spred1; NFLS; hSpred1; spred-1; Sprouty-related; EVH1 domain-containing protein 1; Spred-1; sprouty-related, EVH1 domain containing 1

sprouty-related, EVH1 domain-containing protein 1; Sprouty-related, EVH1 domain-containing protein 1; sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation; protein phosphatase 1, regulatory subunit 147; sprouty-related, EVH1 domain containing 1

SPRED1

SPRED1; SPRED1; NFLS; hSpred1; spred-1; PPP1R147; Spred-1; hSpred1

SPRE1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

444

This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.

Spred1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

Spred1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: Spred1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human Spred1. Buffer: Spred1 Antibody is supplied in PBS containing 0.02% sodium azide.

Spred1 Antibody: The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).

22749221

NP_689807

NM_152594.2

Q7Z699

50,477 Da

Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Kit Receptor Signaling Pathway 198826!!Regulation Of Microtubule Cytoskeleton Pathway 755439!!Signaling Events Mediated By Stem Cell Factor Receptor (c-Kit) Pathway 138068

The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008]

0.1 mg

345 USD