antibody

MICA Antibody

MBS151078

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

MICA Antibody

MICA

MICA; PERB11.1; PERB11.1; MHC class I polypeptide-related sequence A; MIC-A; MHC class I polypeptide-related sequence A

MHC class I polypeptide-related sequence A isoform 1 (MICA*001); MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence A; MICA; HLA class I antigen; stress inducible class I homolog; MHC class I chain-related protein A; MHC class I polypeptide-related sequence A

MICA

MICA; MICA; MIC-A; PERB11.1; MIC-A

MICA_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse

383

MICA Antibody is affinity chromatography purified via peptide column.

Liquid

1mg/mL

MICA antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)

MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Optimal dilutions for each application to be determined by the researcher. Positive Control: 1)MBS151686- A549 Cell Lysate. 2) MBS151716-Human Colon Tissue Lysate

Conjugate: Unconjugated. Immunogen: MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. Buffer: MICA Antibody is supplied in PBS containing 0.02% sodium azide.

MICA Antibody: Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.

4557751

NP_000238

NM_000247.2

Q29983

Predicted: 42 kDa. Observed: 46 kDa

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.33. Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm. Molecular Function: protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding. Biological Process: antigen processing and presentation; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; defense response to bacterium; immune response to tumor cell; response to DNA damage stimulus; gamma-delta T cell activation; defense response to virus; T cell mediated cytotoxicity. Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To

0.1 mg

345 USD