antibody

IDH1 Antibody

MBS151017

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

IDH1 Antibody

IDH1

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; Cytosolic NADP-isocitrate dehydrogenase; IDH; isocitrate dehydrogenase 1 (NADP+), soluble

isocitrate dehydrogenase; Isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase [NADP] cytoplasmic; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; epididymis luminal protein 216; epididymis secretory protein Li 26; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; isocitrate dehydrogenase 1 (NADP+), soluble; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

IDH1

IDH1; IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; PICD; IDH

IDHC_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

414

IDH1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL

IDH1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB)

IDH1 antibody can be used for detection of IDH1 by Western blot at 1 - 2 mug/mL.

Conjugate: Unconjugated. Immunogen: IDH1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human IDH1. Buffer: IDH1 Antibody is supplied in PBS containing 0.02% sodium azide.

IDH1 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production. Defects in IDH1 are involved in the development of glioma.

28178825

NP_005887

NM_005896.3

O75874

46,659 Da

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Abnormal Conversion Of 2-oxoglutarate To 2-hydroxyglutarate Pathway (771598); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Citrate Cycle (TCA Cycle) Pathway (82927)

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Protein type: EC 1.1.1.42; Carbohydrate Metabolism - citrate (TCA) cycle; Other Amino Acids Metabolism - glutathione; Oxidoreductase. Chromosomal Location of Human Ortholog: 2q33.3. Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome; cytosol. Molecular Function: protein homodimerization activity; magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NADP binding; NAD binding; receptor binding. Biological Process: glyoxylate cycle; NADPH regeneration; isocitrate metabolic process; glutathione metabolic process; response to steroid hormone stimulus; tricarboxylic acid cycle; response to oxidative stress; cellular lipid metabolic process; female gonad development; 2-oxoglutarate metabolic process. Disease: Glioma Susceptibility 1

0.1 mg

345 USD