antibody

HVCN1 Antibody

MBS151000

0.1 mg

350 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry

Antibody

HVCN1 Antibody

HVCN1

Hydrogen voltage-gated channel 1; HV1; VSOP; Voltage sensor domain-only protein; (CT) Hydrogen voltage-gated channel 1

voltage-gated hydrogen channel 1 isoform 1; Voltage-gated hydrogen channel 1; voltage-gated hydrogen channel 1; voltage sensor domain-only protein; hydrogen voltage-gated channel 1; Hydrogen voltage-gated channel 1; HV1; Voltage sensor domain-only protein

HVCN1

HVCN1; HVCN1; HV1; VSOP; VSOP; HV1

HVCN1_HUMAN

Polyclonal

Rabbit

Human, mouse, rat

273

Affinity chromatography purified via peptide column

Liquid. PBS containing 0.02% sodium azide.

1 mg/ml

Store at 4 degree C for three months and -20 degree C. Stable for up to one year.

ELISA, Western Blot (WB), Immunohistochemistry (IHC)

HVCN1 antibody can be used for detection of HVCN1 by Western Blot at 0.5 - 1 ug/mL.

Immunogen: 17 amino acid peptide near the carboxy terminus of human HVCN1.

Blocking Peptide: MBS152216 HVCN1 Peptide. Positive Control 1: MBS151674 Spleen Lysate

HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.

* Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6. * Sasaki M, Takagi M, and Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel. Science2006; 312: 589-92. * Morgan D, Capasso M, and Musset B. Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis. Proc. Natl. Acad. Sci. USA2009; 106:18022-7. * Capasso M, Bhamrah MK, Henley T, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat. Immunol.2010;11:265-72.

91992155

NP_115745

NM_032369.3

Q96D96

31,683 Da

This gene encodes a voltage-gated protein channel protein expressed more highly in certain cells of the immune system. Phagocytic cells produce superoxide anions which require this channel protein, and in B cells this same process facilitates antibody production. This same channel protein, however, can also regulate functions in other cells including spermatozoa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function: Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. Ref.5. Enzyme regulation: Sensitive to zinc ions and is inhibited by them. Ref.5. Subcellular location: Membrane; Multi-pass membrane protein Ref.5. Tissue specificity: Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen. Ref.5. Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain. Sequence similarities: Belongs to the hydrogen channel family. Sequence caution: The sequence AAQ89413.1 differs from that shown. Reason: Frameshift at positions 164 and 175.

0.1 mg

350 USD