antibody

ASK1 Antibody

MBS150980

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

ASK1 Antibody

ASK1

ASK1; ASK1; MEKK5; MAPKKK5; ASK1; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; mitogen-activated protein kinase kinase kinase 5

Mitogen-activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5

MAP3K5

MAP3K5; MAP3K5; ASK1; MEKK5; MAPKKK5; ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5

M3K5_HUMAN

Polyclonal

IgG

Rabbit

Human

1374

ASK1 Antibody is affinity chromatography purified via peptide column.

Liquid

ASK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 mug/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Conjugate: Unconjugated. Immunogen: ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. This sequence is different from that of mouse by last two amino acids. Buffer: ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.

ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF- -induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

6685617

Q99683.1

Q99683

155 kDa

Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Modulation And Signaling Pathway 198822!!Cellular Senescence Pathway 905991!!Cellular Responses To Stress Pathway 645258!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020!!Corticotropin-releasing Hormone Pathway 920957!!HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway 137995!!Insulin Signaling Pathway 198845

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

0.1 mg

345 USD